Abstract
A simple, economical, and rapid method has been developed for the determination of the pharmaceutical compound (furosemide) in its pure forms and several pharmaceutical formulations using oxidative coupling reactions. This method focuses on the reaction of the drug compounds with chlorpromazine hydrochloride as a reagent in an acidic medium in the presence of the appropriate oxidizing compound. The absorbance of the colored product was measured at 525 nm and molar absorptivity was 138891 L/mol.cm, compatible with Beer law in concentrations (2-32) μg/ml, the detection, and the quantification limits were (0.471, 1.571) µg/ml respectively, with a recovery rate of 101.15% and the relative standard deviation was less than 0.5%. The great correlation coefficient value (R2 = 0.9953) and insignificant values of intercept (0.067) validated the good linearity of the calibration curve and agreement with Beer’s law. The created method was applied successfully to determine furosemide in pharmaceutical formulation, and the method was in agreement with the standard addition method.