Chemical Problems; 2026; V. 24(4); p. 660-669
SPECTROPHOTOMETRIC DETERMINATION OF DOXORUBICIN HYDROCHLORIDE USING CHARGE TRANSFER COMPLEXES REACTION
Chemistry Department of the College of Education for pure science. University of Mosul, Mosul- Iraq
E-mail:
dr.omar1979@uomosul.edu.iq
Received Date: 2025-05-15
Accepted Date: 2025-07-17
Notes:
Abstract: A new, simple, sensitive, and rapid spectrophotometric method has been developed for the determination of doxorubicin hydrochloride (DOX) via charge-transfer complex formation with o-chloranil in its pure form and pharmaceutical preparations (injection). The method is based on the formation of a stable 2:1 (Reagent: Drug) complex in an ethanol medium, showing maximum absorbance at 531 nm. in order to get the optimal conditions. All factors that might potentially affect the spectrophotometric absorption of the charge-transfer complex formed with doxorubicin were thoroughly examined. Beer’s law was obeyed in the concentration range of 0.1–12 µg/mL with a molar absorptivity of 41148.60 Lꞏmol⁻¹ꞏcm⁻¹ and a detection limit value was of 0.0072 µg/mL while the quantification limit was 0.0220 μg/mL. The proposed method demonstrated excellent accuracy and precision, with average recoveries close to 100%. It was successfully applied to the quantification of doxorubicin in pharmaceutical formulations without interference from excipients. Compared to other methods such as HPLC and fluorometric, this technique offers a cost-effective, rapid, and accessible alternative for routine analysis in quality control.