SPECTROPHOTOMETRIC DETERMINATION OF ROSUVASTATIN CALCIUM WITH VANILLIN REAGENT BY USING ACETYLATION REACTION

Abstact: A sensitive, rapid, and accurate spectrophotometric method was proposed for the determination of rosuvastatin calcium (ROS-Ca) using nucleophilic addition reactions. The acetylation reaction is an important reaction resulting from the reaction of alcohol groups with aldehydes or ketones, which gives products with distinctive colors. This method is based on the acetylation formation from the reaction of forming hydroxyl groups in ROS-Ca with the carbonyl group in the aldehyde (vanillin) in an acidic medium of concentrated sulfuric acid to give a colored product that has a higher absorption at a 550 nm wavelength. The calibration curve gave a linear relationship in the concentration range of 1.25-15 μg/mL. The molar absorptivity was (9×104) L.mol⁻¹.cm⁻¹ and Sandell’s sensitivity index was (0.0011 µg.cm⁻².ml) with a correlation coefficient of 0.9995. The limits of detection (LOD) and quantitation (LOQ) values were 0.0375 and 0.1252 μg/mL, respectively. The method has been successfully applied to pharmaceutical preparation tablets from different origins.