Abstract
This study includes the preparation and characterization of new compound (4-bromo-5-(N-((4- chlorophenyl) (1,1-dioxide-3-oxobenzo isothiazol-2(3H)-yl)methyl)sulfamoyl)-2-((furan- 2-ylmethyl)amino)benzoic acid) from the reaction of furosemide, Saccharin and p-bromo benzaldehyde in a molar ratio 1:1:1 in ethanol. All the complexes are prepared from the reaction of Mannich bases with metal nitrate salts of transition metals such as Co, Ni, Cu and Zn in equimolar ratio (1:1) in ethanol solvent. The prepared compounds were characterized by elemental analysis (C.H.N.S), H-NMR, FT-IR, molar conductivity and magnetic sensitivity were determined. The results showed that furosemide derivatives bi- dentate are coordinated by nitrogen and oxygen atoms with metal, giving tetrahedral geometry. The antibacterial activity was studied for ligands and their complexes using the agar diffusion method. All the prepared compounds were studied and applied to two types of bacteria at different concentrations. It was showed, that the obtained complexes demonstrate a higher inhibitory effect on Staphylococcus aureas bacteria than on Pseudomonas aeruginosa bacteria.